The greatest hits album contains remixes of fifteen of her hit singles from 1983 to 1990, as well as the tracks 'Justify My Love' and 'Rescue Me'. Its title is a loose pun on the Immaculate Conception, the conception of the Virgin Mary without the stain of original sin (Madonna was raised as a Roman Catholic).

Disc 1
1. Holiday - Madonna (4.04)
2. Lucky Star - Madonna (3.39)
3. Borderline - Madonna (4.00)
4. Like a Virgin - Madonna (3.11)
5. Material Girl - Madonna (3.53)
6. Crazy for You - Madonna (3.45)
7. Into the Groove - Madonna (4.10)
8. Live to Tell - Madonna (5.19)
9. Papa Don't Preach - Madonna (4.11)
10. Open Your Heart - Madonna (3.51)
11. La Isla Bonita - Madonna (3.48)
12. Like a Prayer - Madonna (5.51)
13. Express Yourself - Madonna (4.04)
14. Cherish - Madonna (3.52)
15. Vogue - Madonna (5.19)
16. Justify My Love - Madonna (5.00)
17. Rescue Me - Madonna (5.31)